The Effect of Gelam (Melaleuca cajuputi) Honey on Inflammatory Mediators In Periodontitis-Induced Sprague-Dawley Rats.

Saliana A Aziz, Norashikin Hamzah, Afifah-Radiah Fauzi, Yasmin Anum Mohd Yusof, Norliwati Ibrahim, Mariati Abdul Rahman, Norzana Abdul Ghafar, Badiah Baharin

Abstract


Summary. Periodontitis is an inflammatory condition of the tooth supporting structures that will lead to tooth loss. Research has proven that Gelam honey has an anti-inflammatory effect and  potential for treating inflammatory condition. This study aimed to determine the effect of Gelam honey on the inflammatory mediators systemically and locally in Sprague-Dawley rats with periodontitis. Twenty-eight rats were randomly assigned into 4 groups; NLS (control treated with saline), NLH (control treated with Gelam honey, 3g/kg body weight), LS (periodontitis treated with saline), LH (periodontitis treated with Gelam honey, 3g/kg body weight). Periodontitis was induced by ligating the lower left first molar with 4/0  Black Silk Suture. On day 15, plasma and tissue samples were analysed using ELISA and histological staining. The LS group exhibited significantly higher levels of inflammatory cells and IL-1β in the tissues than other groups.  No significant difference of plasma IL-1β levels was found between all of the groups. Gelam honey was able to reduce the level of IL-1β in diseased rats with a total reduction of 21.26% in plasma, 81.27% in immunohistochemical staining. In conclusion, Gelam honey was able to reduce the level of IL-1β in periodontitis rats. Therefore, Gelam honey has potential to be included in dental products especially for treating periodontal disease.

Industrial relevance. Periodontitis is one of the major dental diseases in most of the population in the world. It is an infectious disease and produced an inflammatory condition. Gelam honey (Melaleuca Cajuputi)  is one of the fresh honeys which are easily available in Malaysia and research has proven that Gelam honey has an anti-inflammatory effect. The present study demonstrated that Gelam honey able to reduce inflammatory cells and mediators namely IL-1β in the plasma and in the tissue samples from rats induced with Periodontitis. The results of this study showed that Gelam honey has the potential to be the remedy for treating periodontal disease and this study may be the stepping stone to further the usage of honey in dentistry.

Keywords. Inflammatory mediators; inflammation; honey; periodontitis

Keywords


Inflammatory mediators; inflammation; honey; periodontitis

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